Direct analysis of biogenic amines in water matrix by modified capillary zone electrophoresis with 18-crown-6

• DOI: 10.1007/s00604-011-0755-4
• Publication Date: 2012-01-07T05:51:09Z
• Authors: Wen-li Li; Jin-yuan Ge; Ya-li Pan; Qing-cui Chu; Jian-nong Ye
• Keywords: 0404 agricultural biotechnology; 04 agricultural and veterinary sciences; 6. Clean water

We report on a method for the determination of the biogenic amines (BAs) spermine, spermidine, histamine, cadaverine, β-phenylethylamine, tyramine and tryptamine. It is based on capillary zone electrophoresis in the presence of 18-crown-6 (180 mM), and is making use of amperometric detection. Under optimized conditions, seven BAs could be well separated within 29 min at a separation voltage of 14 kV in a buffer solution of pH 3.6. The limits of detection for seven BAs are around 10 ng.mL−1 for standard mixtures. The method does not require preconcentration and derivatization steps, and thus provides an attractive alternative to quantitative multi-analysis of BAs in water samples.