Plaque assay for African swine fever virus on swine macrophages

• DOI: 10.1007/s00705-002-0814-6
• Publication Date: 2003-03-05T16:20:13Z
• Authors: M. J. Bustos; M. L. Nogal; Y. Revilla; A. L. Carrascosa
• Keywords: 0301 basic medicine; 03 medical and health sciences; Swine; Macrophages, Alveolar; Animals; Viral Plaque Assay; African Swine Fever Virus; Cells, Cultured; 3. Good health

A plaque assay developed to detect the infection of African Swine Fever Virus on swine macrophages is described. Plaques were generated by all of the virus isolates tested. The method is suitable not only for virus titration but also for the selection of clones in protocols for isolation/purification of recombinant viruses.