A Chinese isolate of barley yellow dwarf virus-PAV represents a third distinct species within the PAV serotype

• DOI: 10.1007/s00705-007-0947-8
• Publication Date: 2007-03-08T11:38:32Z
• Authors: F. Liu; X. Wang; Y. Liu; J. Xie; S. M. Gray; G. Zhou; B. Gao
• Keywords: 0301 basic medicine; China; Base Sequence; Sequence Homology, Amino Acid; Molecular Sequence Data; Hordeum; 3. Good health; 03 medical and health sciences; Species Specificity; Sequence Homology, Nucleic Acid; DNA, Viral; Luteovirus; Nucleic Acid Conformation; Capsid Proteins; Amino Acid Sequence; Serotyping; 3' Untranslated Regions; Phylogeny; DNA Primers; Plant Diseases

The complete nucleotide sequence of barley yellow dwarf virus (BYDV) PAV-CN genomic RNA was determined. This represents the seventh complete genome sequence of a BYDV-PAV serotype. The genome organization of PAV-CN was comparable to that of other BYDV-PAV serotypes, but the nucleotide sequence of full genome was only 76.9-80.3% similar. Sequence similarity of individual open reading frames and untranslated regions (UTR) between PAV-CN and other PAV isolates ranged from 37.9 to 98.2%. Overall, PAV-CN was most similar to BYDV-PAS, which belongs to one of two distinct species within the PAV serotype of BYDV, although the 5' UTR and ORF1 of PAV-CN was most similar to BYDV-GAV, another member of the genus Luteovirus that is not serologically related to BYDV-PAV. These data suggest that PAV-CN may have undergone a recombination event with GAV and that PAV-CN represents a third distinct species within the PAV serotype of BYDV.