Nucleotide sequence and genome organization of Dweet mottle virus and its relationship to members of the family Betaflexiviridae

• DOI: 10.1007/s00705-010-0758-1
• Publication Date: 2010-07-19T18:35:09Z
• Authors: Subhas Hajeri; Chandrika Ramadugu; Manjunath Keremane; Georgios Vidalakis; Richard Lee
• Keywords: ORFS; Genomic Organization

The nucleotide sequence of Dweet mottle virus (DMV) was determined and compared to sequences members the families Alphaflexiviridae Betaflexiviridae. DMV genome has 8,747 nucleotides (nt) excluding 3' poly-(A) tail. genomic RNA contains three putative open reading frames (ORFs) untranslated regions 73 nt at 5' 541 termini. ORF1 potentially encoding a 227.48-kDa polyprotein, which methyltransferase, oxygenase, endopeptidase, helicase, RNA-dependent polymerase (RdRP) domains. ORF2 encodes movement protein 40.25 kDa, while ORF3 coat 40.69 kDa. Protein database searches showed 98-99% matches ORFs with citrus leaf blotch (CLBV) sequences. Phylogenetic analysis based on RdRP core domain revealed that is closely related CLBV as member genus Citrivirus. did not satisfy molecular criteria for demarcation an independent species within Citrivirus, family Betaflexiviridae, hence, can be considered isolate.