Complete nucleotide sequence of a begomovirus and associated betasatellite infecting croton (Croton bonplandianus) in Pakistan

• DOI: 10.1007/s00705-011-0993-0
• Publication Date: 2011-04-12T10:24:52Z
• Authors: Khadim Hussain; Mazhar Hussain; Shahid Mansoor; Rob W. Briddon
• Keywords: Croton; Leaf curl; Geminiviridae; Sequence (biology)

The complete sequences of a begomovirus and an associated betasatellite isolated from Croton bonplandianus originating from Pakistan were determined. The sequence of the begomovirus showed the highest level of nucleotide sequence identity (88.9%) to an isolate of papaya leaf curl virus and thus represents a new species, for which we propose the name Croton yellow vein virus (CYVV). The sequence of the betasatellite showed the highest levels of sequence identity (82 to 98.4%) to six sequences in the databases that have yet to be reported, followed by isolates of tomato leaf curl Joydebpur betasatellite (48.7 to 52.5%). This indicates that the betasatellite identified here (and the six sequences in the databases) is an isolate of a newly identified species for which the name Croton yellow vein mosaic betasatellite (CroYVMB) is proposed. For the begomovirus, an analysis of the sequence indicates that it has a recombinant origin.