Complete genome sequence of motherwort yellow mottle virus, a novel putative member of the genus Torradovirus

• DOI: 10.1007/s00705-014-2254-5
• Publication Date: 2014-10-09T17:04:31Z
• Authors: Jang-Kyun Seo; Minji Kang; Hae-Ryun Kwak; Mi-Kyeong Kim; Chang-Seok Kim; Su-Heon Lee; Jeong-Soo Kim; Hong-Soo Choi
• Keywords: ORFS

The complete genome sequence of a new virus isolated from a motherwort plant exhibiting yellow mottle, mild mosaic, and stunting symptoms in Andong, Korea, was determined. The genome of this virus is composed of two single-stranded RNAs (7068 and 4963 nucleotides in length, respectively) carrying poly(A) tails. RNA1 contains one large open reading frame (RNA1-ORF1), while two potential ORFs (RNA2-ORF1 and RNA2-ORF2) were found in RNA2. BLAST searches of protein databases showed that RNA1-ORF1 and RNA2-ORF2 have maximum amino acid sequence identities of 53 % and 57 % to the RNA1-ORF1 and RNA2-ORF2, respectively, of lettuce necrotic leaf curl virus (LNLCV, a recently identified torradovirus). Phylogenetic analysis provided further evidence that the virus identified in this study is probably a member of a new species in the genus Torradovirus. The name "motherwort yellow mottle virus" (MYMoV) is proposed for this new virus.