Complete genome sequence of Tacaribe virus

• DOI: 10.1007/s00705-020-04681-9
• Publication Date: 2020-05-27T19:02:30Z
• Authors: Julia Holzerland; Anne Leske; Lucie Fénéant; Dominique Garcin; Daniel Kolakofsky; Allison Groseth
• Keywords: Arenavirus; Viral quasispecies; Intergenic region; Sequence (biology)

Tacaribe virus (TCRV) is the prototype of New World arenaviruses (also known as TCRV serocomplex viruses). While not itself a human pathogen, many closely related members this group cause hemorrhagic fever, and thus has long served an important BSL2 system for research into diverse areas arenavirus biology. Due to its widespread use, coding-complete sequence both S L segments bipartite genome been publically available almost 30 years. However, more recently, found contain significant discrepancies compared other samples same original strain (i.e., TRVL-11573). Further, it incomplete with respect ends, which critical regulatory elements RNA synthesis. In order rectify these issues we now present first complete arenavirus. addition completing segment 5' end, identified apparent error in 3' end well substantial intergenic region likely affect folding. Comparison existing partial sequences confirmed 12-amino-acid deletion GP, including putative glycosylation sites, 4-amino-acid exchange flanking exonuclease domain NP. Accounting corrections, TRVL-11573 appears be nearly identical that isolated Florida 2012. The availability information provides solid basis future molecular genetic work on