Antimicrobial and antitumor properties of anuran peptide temporin-SHf induce apoptosis in A549 lung cancer cells

• DOI: 10.1007/s00726-023-03373-3
• Publication Date: 2024-02-06T10:02:10Z
• Authors: Anet Antony; Anupama Kizhakke ...; Shilpa Olakkaran; Shweta Dhannura; Shamasoddin Shekh; Konkallu Hanumae ...; Hunasanahally Put...
• Keywords: 0301 basic medicine; Lung Neoplasms; QH301-705.5; Cytotoxicity; Apoptosis; QD415-436; Biochemistry; Temporin-SHf; 03 medical and health sciences; Anti-Infective Agents; Humans; Animals; A549 cells; Original Article; Angiogenesis; Biology (General); Anura; Anticancer peptides; Antimicrobial Cationic Peptides

Abstract Temporin-SHf is a linear, ultra-short, hydrophobic, α-helix, and phe-rich cationic antimicrobial peptide. The antitumor activities mechanism of temporin-SHf-induced cancer cell death are unknown. temporin-SHf was synthesized by solid-phase Fmoc chemistry were investigated. microbiocidal, non-hemolytic, cytotoxic to human cells but not non-tumorigenic cells. It affected the cells' lysosomal integrity caused membrane damage. inhibited A549 proliferation migration. anti-angiogenic causes through apoptosis. molecular action confirmed that it kills triggering caspase-dependent apoptosis an intrinsic mitochondrial pathway. Owing its short length broad spectrum activity, promising candidate for developing new class anticancer drugs.