Protein adsorption/desorption dynamics on Ca-enriched titanium surfaces: biological implications

• DOI: 10.1007/s00775-021-01886-4
• Publication Date: 2021-08-27T18:02:36Z
• Authors: Francisco Romero-...; Andreia Cerqueira; Eduardo Anitua; Ricardo Tejero; Iñaki García-Arnáez; Cristina Martinez...; Seda Ozturan; Raul Izquierdo; Mikel Azkargorta; Félix Elortza; Mariló Gurruchaga; Isabel Goñi; Julio Suay
• Keywords: Dynamics; Protein Adsorption

Abstract Calcium ions are used in the development of biomaterials for promotion coagulation, bone regeneration, and implant osseointegration. Upon implantation, time-dependent release calcium from titanium surfaces modifies physicochemical characteristics at implant–tissue interface thus, biological responses. The aim this study is to examine how dynamics protein adsorption on these change over time. Titanium discs with without Ca were incubated human serum 2 min, 180 960 min. layer proteins attached surface was characterised using nLC-MS/MS. kinetics different between materials, revealing an increased associated coagulation immune responses prior release. Implant–blood contact experiments confirmed strong coagulatory effect surfaces. We employed primary alveolar osteoblasts THP-1 monocytes osteogenic inflammatory In agreement proteomic results, Ca-enriched showed a significant initial inflammation that disappeared once released. distinct adsorption/desorption found work demonstrated be useful explain differential Ca-ion modified Graphic abstract