Stability of the H-cluster under whole-cell conditions—formation of an Htrans-like state and its reactivity towards oxygen

• DOI: 10.1007/s00775-022-01928-5
• Publication Date: 2022-03-08T08:02:38Z
• Authors: Marco Lorenzi; Pierre Ceccaldi; Patricia Rodrígue...; Holly Jayne Redman; Afridi Zamader; James A. Birrell; Livia S. Mészáros; Gustav Berggren
• Keywords: Hydrogenase; Reactivity; Molecular oxygen; Iron–sulfur cluster

Hydrogenases are metalloenzymes that catalyze the reversible oxidation of molecular hydrogen into protons and electrons. For this purpose, [FeFe]-hydrogenases utilize a hexanuclear iron cofactor, H-cluster. This biologically unique cofactor provides enzyme with outstanding catalytic activities, but it is also highly oxygen sensitive. Under in vitro conditions, stable forms H-cluster denoted H