The concurrent stimulation of Wnt and FGF8 signaling induce differentiation of dental mesenchymal cells into odontoblast-like cells

• DOI: 10.1007/s00795-021-00297-3
• Publication Date: 2021-11-05T12:02:32Z
• Authors: Motoyoshi Kimura; Akiko Saito; Shoko Onodera; Takashi Nakamura; Makoto Suematsu; Seikou Shintani; Toshifumi Azuma
• Keywords: DMP1; Dentin sialophosphoprotein; FGF8; Dental follicle

Fibroblast growth factor 8 (FGF8) is known to be a potent stimulator of canonical Wnt/β-catenin activity, an essential for tooth development. In this study, we analyzed the effects co-administration FGF8 and CHIR99021 (GSK3β inhibitor) on differentiation dental mesenchymal cells into odontoblasts. Utilizing Cre-mediated EGFP reporter mice, dentin matrix protein 1 (Dmp1) expression was examined in mouse neonatal molar germs. At birth, Dmp1-EGFP not found but rather epithelial cells, after which Dmp1-positive gradually emerged area along with disappearance area. Primary cultured from germ specimens showed loss positive signals, whereas addition Wnt3a or significantly regained Dmp1 positivity within approximately 2 weeks. Other odontoblast markers such as sialophosphoprotein (Dspp) could clearly detected. Concurrent stimulation primary resulted significant upregulation odonto/osteoblast proteins. Furthermore, increased levels runt-related transcription (Runx2), osterix, osteocalcin were also observed. The present findings indicate that coordinated action signals germs mice.