In vitro removal of deoxynivalenol and T-2 toxin by lactic acid bacteria

• DOI: 10.1007/s10068-012-0223-x
• Publication Date: 2013-01-03T15:35:00Z
• Authors: Zhong-Yi Zou; Zhi-Fei He; Hong-Jun Li; Peng-Fei Han; Xiao Meng; Yu Zhang; Fang Zhou; Ke-Pei Ouyang; Xi-Yue Chen; Jun Tang
• Keywords: Biotransformation; Incubation period

Five strains of lactic acid bacteria were tested for their ability to remove deoxynivalenol (DON) and T-2 toxin from MRS broth. The ability of Lactobacillus plantarum strain 102 (LP102) was the strongest among 5 strains after incubation at 37°C for 72 h. The mode of removal was physical binding, rather than biotransformation. The abilities were not significantly different between when removing single toxin and when removing mixed toxins by viable cells of LP102. DON and T-2 toxin released from LP102 viable cell-toxin complexes were 28.22±1.55 and 35.42±2.02% of total bound toxins respectively after 3 times of wash with posphate buffered saline, respectively, those were 4.59±0.86 and 5.59±1.47% after incubation with simulated gastric fluid (SGF) at 37oC for 4 h, and 6.86±0.81 and 9.04±1.13% after incubation with simulated intestinal fluid (SIF) at 37oC for 4 h, respectively.