The effect of berberine chloride and/or its combination with vancomycin on the growth, biofilm formation, and motility of Clostridioides difficile

• DOI: 10.1007/s10096-020-03857-0
• Publication Date: 2020-07-08T10:03:43Z
• Authors: Dorota Wultańska; Michał Piotrowski; Hanna Pituch
• Keywords: Minimum bactericidal concentration; Clostridioides

Abstract This study aims to investigate the antimicrobial and antibiofilm activity of berberine chloride (BBR) vancomycin (VAN) as well synergistic combinations BBR with VAN against Clostridioides difficile strains. The effect different concentrations on strain motility was also assessed. Twelve C. strains (two reference 630, ATCC 9689, one control M120, 9 clinical belonging PCR-ribotype (RT027)) were collected investigated for their susceptibility in planktonic biofilm forms. Both minimum inhibitory concentration (MIC) bactericidal (MBC) found vary over a broad range (256–1.024 mg/L 256–16.384 mg/L, respectively). MIC MBC varied greatly, ranging from 0.25 4.0 64.0 MBC. sub-MIC (1/2 MIC) reduced MICs forms ten enhanced formation be statistically significant. In addition, surprisingly strain. inhibition presence significant 3 ( p < 0.05). Altogether, exhibited strong , analysis combination showed effect.