Photobiomodulation with 808-nm diode laser enhances gingival wound healing by promoting migration of human gingival mesenchymal stem cells via ROS/JNK/NF-κB/MMP-1 pathway

• DOI: 10.1007/s10103-020-03040-z
• Publication Date: 2020-05-26T00:02:33Z
• Authors: Jie Feng; Xiaoyan Li; Siying Zhu; Yongmei Xie; Juan Du; Huabing Ge; Yuxing Bai; Yi Liu; Lijia Guo
• Keywords: 0301 basic medicine; Wound Healing; MAP Kinase Signaling System; Gingiva; JNK Mitogen-Activated Protein Kinases; NF-kappa B; Mesenchymal Stem Cells; Mitochondria; 03 medical and health sciences; Cell Movement; Humans; Lasers, Semiconductor; Matrix Metalloproteinase 1; Phosphorylation; Reactive Oxygen Species

Photobiomodulation (PBM) has been shown to improve wound healing by promoting mesenchymal stem cell migration and proliferation. However, it remains unknown whether an 808-nm diode laser can influence human gingival mesenchymal stem cells (HGMSCs), and which dose this works well. In the present study, it was found that PBM could promote the migration of HGMSCs but not the proliferation. Furthermore, PBM could activate mitochondrial ROS, which could elevate the phosphorylation levels of JNK and IKB in HGMSCs, and further activate NF-κB as the nuclear translocation of p65 is elevated. Taken together, these present results indicate that PBM might promote cell migration via the ROS/JNK/NF-κB pathway.