Production of Homozygous Transgenic Rainbow Trout with Enhanced Disease Resistance

• DOI: 10.1007/s10126-013-9550-z
• Publication Date: 2013-10-01T04:09:28Z
• Authors: Pinwen Peter Chiou; Maria J. Chen; Chun-Mean Lin; Jenny Khoo; Jon Larson; Rich Holt; Jo-Ann Leong; Gary Thorgarrd; Thomas T. Chen
• Keywords: Disease resistant

Previous studies conducted in our laboratory showed that transgenic medaka expressing cecropin B transgenes exhibited resistant characteristic to fish bacterial pathogens, Pseudomonas fluorescens and Vibrio anguillarum. To confirm whether antimicrobial peptide gene will also exhibit anti-bacterial anti-viral characteristics aquaculture important species, we produced rainbow trout P1 or a synthetic analog, CF-17, transgene by sperm-mediated transfer method. About 30 % of recovered from electroporation were shown carry the as determined polymerase chain reaction (PCR) amplification assay. Positive P₁ crossed non-transgenic establish F₁ founder families, subsequently generating F₂, F₃ progeny. Expression CF-17 was detected reverse transcription (RT)-PCR analysis. The distribution body sizes among not significantly different those fish. Results challenge revealed many families F₂ resistance infection Aeromonas salmonicida infectious hematopoietic necrosis virus (IHNV). All-male homozygous androgenesis sperm heterozygous one generation. A. confirmed progeny derived outcross all-male females. current possibility producing disease-resistant strains transgenesis followed androgenesis.