Quantitative imaging of directional transport through plasmodesmata in moss protonemata via single-cell photoconversion of Dendra2

• DOI: 10.1007/s10265-013-0547-5
• Publication Date: 2013-02-04T14:20:04Z
• Authors: Munenori Kitagawa; Tomomichi Fujita
• Keywords: Plasmodesma; Protonema; Physcomitrella patens

Abstract Cell-to-cell transport of molecules in plants must be properly regulated for plant growth and development. One specialized mechanism that have evolved involves through plasmodesmata (PD), but when how via PD is among individual cells remains largely unknown, particularly at the single-cell level. Here, we developed a tool quantitatively analyzing cell-to-cell level using protonemata Physcomitrella patens photoconvertible fluorescent protein, Dendra2. In filamentous protonemal tissues, one-dimensional intercellular communication can observed easily. Using this system, found Dendra2 was directionally transported toward apex growing protonemata. However, directional could eliminated by incubation dark or treatment with metabolic inhibitor. Thus, propose macromolecules occur moss protonemata, may affected photosynthetic activity cells.