Metabolic engineering ofEscherichia colito produce succinate from woody hydrolysate under anaerobic conditions

• DOI: 10.1007/s10295-020-02259-7
• Publication Date: 2020-01-27T09:02:55Z
• Authors: Fayin Zhu; Chengqiang Wang; Ka-Yiu San; George N Bennett
• Keywords: Metabolic Engineering; Succinic acid; Polyhydroxybutyrate

AbstractIt is of great economic interest to produce succinate from low-grade carbon sources, e.g., lignocellulosic biomass hydrolysate, which mainly contains glucose and xylose. Inactivation of the glucose uptake system PtsG was evaluated for succinate production from xylose-rich feedstocks. Strains with integration of succinate production modules into the chromosome of Escherichia coli were then constructed. These strains have better succinate production performance from xylose-rich feedstocks than strain FZ560 harboring pHL413KF1. Glucose utilization was enhanced in FZ661T by manipulation of the gal operon to allow efficient use of the high-concentration glucose in woody biomass hydrolysate. Up to 906.7 mM (107.0 g/L) succinate was produced from mixed sugars in fed-batch fermentation and more than 461.7 mM (54.5 g/L) succinate was produced from woody hydrolysate in a batch fermentation. In this study, FZ661T was able to produce succinate from woody hydrolysate in minimal medium efficiently, making it attractive for industrial applications in succinate production.