Haladaptatus pallidirubidus sp. nov., a halophilic archaeon isolated from saline soil samples in Yunnan and Xinjiang, China

• DOI: 10.1007/s10482-014-0259-4
• Publication Date: 2014-09-04T22:15:12Z
• Authors: Bing-Bing Liu; Wan-Yu Zhao; Xiao Chu; Wael N. Hozzein; Deene Manik Prabhu; Mohammed A. M. Wa...; Shu-Kun Tang; Li-Li Zhang; Wen-Jun Li
• Keywords: Microbiological Techniques; 0301 basic medicine; Base Composition; China; Molecular Sequence Data; Nucleic Acid Hybridization; Sequence Analysis, DNA; Hydrogen-Ion Concentration; Sodium Chloride; 15. Life on land; Archaea; DNA, Ribosomal; Aerobiosis; 03 medical and health sciences; Cytosol; DNA, Archaeal; Microscopy, Electron, Transmission; RNA, Ribosomal, 16S; Cluster Analysis; Glycolipids; Phospholipids; Phylogeny; Soil Microbiology

Two extremely halophilic archaea, designated YIM 90917 and YIM 93656(T), were isolated from saline soils in Yunnan province and Lup nur region in Xinjiang province, western China, respectively. Colonies of the two strains were observed to be pink-pigmented. The cells were found to be Gram-stain negative, coccoid and non-motile. The organisms were found to be aerobic and could grow in an NaCl range of 6-35 % (optimum 18 %), temperatures ranging from 25 to 50 °C (optimum 37-42 °C), pH range from 6.0-8.5 (optimum pH 7.0-7.5) and Mg(2+) range from 0 to 1.5 M (optimum 0.5-1.0 M); Mg(2+) was not necessary for growth. Cells were not observed to lyse in distilled water. Strains YIM 90917 and YIM 93656(T) showed the highest 16S rRNA gene sequence similarities to Haladaptatus cibarius JCM 15962(T) (97.6 and 97.9 %, respectively). In addition, the DNA-DNA hybridizations of strains YIM 90917 and YIM 93656(T) with type strains H. cibarius JCM 15962(T), Haladaptatus litoreus JCM 15771(T) and Haladaptatus paucihalophilus JCM 13897(T) were 37.2 and 38.2 %, 36.6 and 39.0 % and 27.9 and 27.7 %, respectively. The DNA G+C contents of strains YIM 90917 and YIM 93656(T) were determined to be 56.0 and 57.4 mol%. The major polar lipids of the two strains were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, sulfated mannosyl glucosyl diether and other four unidentified glycolipids. On the basis of physiological, chemotaxonomic data and phylogenetic analysis, the strains YIM 90917 and YIM 93656(T) can be classified as a novel species of the genus Haladaptatus, for which the name Haladaptatus pallidirubidus sp. nov. is proposed. The type strain is YIM 93656(T) (=JCM 17504(T) = CCTCC AB2010454(T)).