Description and genomic characterization of Streptococcus symci sp. nov., isolated from a child’s oropharynx

• DOI: 10.1007/s10482-020-01505-3
• Publication Date: 2021-01-02T15:02:57Z
• Authors: He Qi; Defeng Liu; Yang Zou; Nan Wang; Han Tian; Chunling Xiao
• Keywords: Coccus (insect); Streptococcus bovis

Using the culturomics approach, we isolated a new Streptococcus species, strain C17T, from oropharynx mucosa sample of healthy 5-year-old child living in Shenyang, China. We studied phenotypic, phylogenetic, and genomic characteristics which was identified as Gram-positive, coccus-shaped, non-motile, aerobic, catalase-negative bacteria. Its growth temperatures ranged 20 to 42 °C, with optimal at 37 °C. Acid production could be inhibited by two sugars, trehalose raffinose. In reactions for enzyme lipase (C14) were confirmed negative, whereas those alkaline phosphatase, α-glucosidase, hippuric acid hydrolysis positive. The C17T genome contained 2,189,419 base pairs (bp), an average G+C content 39.95%, encoding 2092 genes total. 16S ribosomal RNA sequence showed 99.8% similarity newly pseudopneumoniae ATCC BAA-960T. main fatty components C16:0, C18:1 w7c, C18:0, w9c, all can found other species genus. Strain high susceptibility clindamycin, linezolid, vancomycin, chloramphenicol, cefepime, moderate erythromycin. obtained dDDH value between closest 52.9%. addition, whole had 82.21-93.40% nucleotide identity (ANI) strains closely related indicating that unique among species. Based on these characteristics, determine is novel named symci sp. nov. (= GDMCC 1.1633 = JCM 33582).