TRIM16 overexpression induces apoptosis through activation of caspase-2 in cancer cells

• DOI: 10.1007/s10495-013-0813-y
• Publication Date: 2013-02-12T03:59:40Z
• Authors: Patrick Y. Kim; Aldwin Suryo Rahm...; Owen Tan; Murray D. Norris; Michelle Haber; Glenn M. Marshall; Belamy B. Cheung
• Keywords: 0301 basic medicine; Cancer Research; Clinical Biochemistry; Pharmaceutical Science; Apoptosis; Tripartite Motif Proteins; Neuroblastoma; 2.1 Biological and endogenous factors; anzsrc-for: 31 Biological Sciences; RNA, Small Interfering; Lung; Cancer; Membrane Potential, Mitochondrial; Enzyme Precursors; Tumor; Lung Cancer; anzsrc-for: 3101 Biochemistry and Cell Biology; Caspase 2; Mitochondrial; Mitochondria; 3. Good health; DNA-Binding Proteins; Gene Expression Regulation, Neoplastic; anzsrc-for: 111201 Cancer Cell Biology; Cysteine Endopeptidases; Organ Specificity; anzsrc-for: 0601 Biochemistry and Cell Biology; Signal Transduction; 570; Ubiquitin-Protein Ligases; 610; 3101 Biochemistry and Cell Biology; Small Interfering; Membrane Potential; Cell Line; anzsrc-for: 1116 Medical Physiology; 03 medical and health sciences; Rare Diseases; Cell Line, Tumor; Breast Cancer; Humans; Pharmacology; Biochemistry, medical; Neoplastic; Original Paper; anzsrc-for: 111403 Paediatrics; Neurosciences; Cell Biology; HEK293 Cells; Gene Expression Regulation; Women's Health; RNA; 31 Biological Sciences; Transcription Factors

TRIM16 exhibits tumour suppressor functions by interacting with cytoplasmic vimentin and nuclear E2F1 proteins in neuroblastoma squamous cell carcinoma cells, reducing migration replication. Reduced expression a range of human primary malignant tissues correlates increased potential. also induces apoptosis breast lung cancer unknown mechanisms. Here we show that overexpression (MCF7) (BE(2)-C) but not non-malignant HEK293 cells. procaspase-2 protein levels MCF7 induced caspase-2 activity both BE(2)-C We directly interact cells co-localise Most importantly, the induction is required for to initiate apoptosis. Our data suggest novel mechanism which can promote modulating activity.