Thyroid hormones improve cardiac function and decrease expression of pro-apoptotic proteins in the heart of rats 14 days after infarction

• DOI: 10.1007/s10495-015-1204-3
• Publication Date: 2015-12-12T07:21:02Z
• Authors: Alexandre Luz de ...; Rafael Oliveira F...; Vanessa D. Ortiz; Cristina Campos; Jéssica H. P. Bon...; Tânia R. G. Ferna...; Adriana Conzatti; Rafaela Siqueira; Angela Vicente Ta...; Paulo Cavalheiro ...; Adriane Belló-Klein; Alex Sander da Ro...
• Keywords: Male; 0301 basic medicine; Cardiotonic Agents; Myocardium; Drug Evaluation, Preclinical; Myocardial Infarction; Gene Expression; 3. Good health; Oxidative Stress; Thyroxine; 03 medical and health sciences; Ventricular Pressure; Animals; Triiodothyronine; Lipid Peroxidation; Rats, Wistar; Apoptosis Regulatory Proteins; Reactive Oxygen Species; Oxidation-Reduction

Apoptosis is a key process associated with pathological cardiac remodelling in early-phase post-myocardial infarction. In this context, several studies have demonstrated an anti-apoptotic effect of thyroid hormones (TH). The aim of this study was to evaluate the effects of TH on the expression of proteins associated with the apoptotic process 14 days after infarction. Male Wistar rats (300-350 g) (n = 8/group) were divided into four groups: Sham-operated (SHAM), infarcted (AMI), sham-operated + TH (SHAMT) and infarcted + TH (AMIT). For 12 days, the animals received T3 and T4 [2 and 8 µg/(100 g day)] by gavage. After this, the rats were submitted to haemodynamic and echocardiographic analysis, and then were sacrificed and the heart tissue was collected for molecular analysis. Statistical analyses included two-way ANOVA with Student-Newman-Keuls post test. Ethics Committee number: 23262. TH administration prevented the loss of ventricular wall thickness and improved cardiac function in the infarcted rats 14 days after the injury. AMI rats presented an increase in the pro-apoptotic proteins p53 and JNK. The hormonal treatment prevented this increase in AMIT rats. In addition, TH administration decreased the Bax:Bcl-2 ratio in the infarcted rats. TH administration improved cardiac functional parameters, and decreased the expression of pro-apoptotic proteins 14 days after myocardial infarction.