Ridostin Induces Transcription of a Wide Spectrum of Interferon Genes in Human Cells

• DOI: 10.1007/s10517-013-2313-z
• Publication Date: 2013-12-12T02:11:22Z
• Authors: T. M. Sokolova; A. N. Shuvalov; M. V. Telkov; L. V. Kolodyazhnaya; F. I. Ershov
• Keywords: Adult; 0301 basic medicine; Maus Elberfeld virus; Interferon Inducers; Gossypol; Interferon-alpha; Glycerolphosphate Dehydrogenase; RNA, Fungal; Interferon-beta; Fibroblasts; Interferon alpha-2; Antiviral Agents; Actins; Cell Line; Interferon-gamma; 03 medical and health sciences; 2',5'-Oligoadenylate Synthetase; Humans; Interferons; Lymphocytes; Child; RNA, Double-Stranded

The effects of Ridostin on the transcription of IFN family genes in human fibroblasts and lymphocytes were studied by quantitative real-time PCR. The degree of gene induction by Ridostin was most pronounced in fibroblasts, and was significantly higher than the induction by Kagocel: transcription of IFN-β, oligoadenylate synthetase, and double-stranded RNA-dependent protein kinase genes increased by about 2000, 100, and 20 times, respectively. In lymphocytes, Ridostin also activated a wide variety of IFN family genes, including genes of IFN-β, IFN-γ, and IFN-dependent enzymes, but this induction was less pronounced than in the fibroblasts. It was shown that gene response in lymphocyte from a child with cancer is reduced in comparison with that of adult healthy participant. Ridostin, and even more so Reaferon up-regulated activities of β-actin, glycerophosphate dehydrogenase, and β2-microglobulin genes, thus making impossible or limiting their use as constitutive stable reference genes (standards) in PCR-assays of IFN and their inductors.