Comparison of the Efficiency of Transplantation of Bone Marrow Multipotent Mesenchymal Stromal Cells Cultured under Normoxic and Hypoxic Conditions and Their Conditioned Media on the Model of Acute Lung Injury

• DOI: 10.1007/s10517-014-2509-x
• Publication Date: 2014-06-10T23:28:22Z
• Authors: R. K. Chailakhyan; A. V. Aver’yanov; F. G. Zabozlaev; P. A. Sobolev; A. V. Sorokina; D. A. Akul’shin; Yu. V. Gerasimov
• Keywords: Lipopolysaccharides; Male; 0303 health sciences; Interleukin-6; Acute Lung Injury; Interleukin-1beta; Bone Marrow Cells; Mesenchymal Stem Cells; Mesenchymal Stem Cell Transplantation; Cell Hypoxia; Rats; Oxygen; 03 medical and health sciences; Antigens, CD; Culture Media, Conditioned; Animals; Transplantation, Homologous; Rats, Wistar; Hypoxia; Biomarkers; Cells, Cultured; Injections, Intraperitoneal

The therapeutic efficiency of intravenous injection of rat bone marrow multipotent mesenchymal stromal cells grown under conditions of normoxia and hypoxia (3% O2) and conditioned media from these cultures were compared on the rat model of acute lung injury induced by intraperitoneal injection of lipopolysaccharide. The best therapeutic efficiency was demonstrated by cells grown under hypoxic conditions. The effect of conditioned media was less pronounced and did not depend on the culturing conditions.