Effects of Inhibitors of Necroptosis and Autophagy on Morphofunctional Characteristics of the Myocardium during Static Cold Storage of Donor Rat Heart

• DOI: 10.1007/s10517-015-3078-3
• Publication Date: 2015-10-31T00:01:46Z
• Authors: Yu. V. Dmitriev; S. M. Minasyan; L. V. Vasina; E. A. Demchenko; M. M. Galagudza
• Keywords: Cryopreservation; Male; 0303 health sciences; Cardiotonic Agents; Indoles; Myocardium; Imidazoles; Heart; Organ Size; Tissue Donors; Potassium Chloride; Rats; Cold Temperature; Necrosis; 03 medical and health sciences; Glucose; Autophagy; Animals; Mannitol; Tissue Preservation; Rats, Wistar; Procaine

Cardioprotective effects of necrostatin-1 (necroptosis inhibitor) and 3-methyladenine (autophagy inhibitor) were studied on the model of long-term cold preservation of rat heart. Addition of necrostatin-1 (490 nmol/liter) or 3-methyladenine (4.5 mmol/liter) to custodiol preserving solution reduced myocardial infarction size and improved left-ventricular function during reperfusion after 8-h preservation at 4°C. Inhibition of necroptosis and autophagy contributed to the cardioprotective effect under conditions of cold preservation of the donor heart.