Analysis of Reparative Activity of Platelet Lysate: Effect on Cell Monolayer Recovery In Vitro and Skin Wound Healing In Vivo

• DOI: 10.1007/s10517-016-3563-3
• Publication Date: 2016-11-23T22:40:39Z
• Authors: N. S. Sergeeva; Ya. D. Shanskii; I. K. Sviridova; P. A. Karalkin; V. A. Kirsanova; S. A. Akhmedova; A. D. Kaprin
• Keywords: Blood Platelets; Male; Wound Healing; 0303 health sciences; Platelet-Rich Plasma; Primary Cell Culture; Mesenchymal Stem Cells; Complex Mixtures; Fibroblasts; Rats; 03 medical and health sciences; Cell Movement; Animals; Humans; Biological Assay; Cell Proliferation; Skin

Platelet lysate prepared from donor platelet concentrate and pooled according to a developed technique stimulates migration of multipotent mesenchymal stromal cells of the human adipose tissue and promotes healing of the monolayer defect in cultures of human fibroblasts and multipotent mesenchymal stromal cells in vitro in concentrations close those of fetal calf serum (5-10%). Lysate of platelets from platelet-rich rat blood plasma stimulated healing of the skin defect by promoting epithelialization and granulation tissue formation. The regenerative properties of platelet lysate in vivo increased with increasing its concentration.