Effect of 30-Day Hindlimb Unloading and Hypergravity on Bone Marrow Stromal Progenitors in C57Bl/6N Mice

• DOI: 10.1007/s10517-018-4301-9
• Publication Date: 2018-11-12T01:32:53Z
• Authors: E. A. Markina; I. V. Andrianova; A. S. Shtemberg; L. B. Buravkova
• Keywords: Hypergravity; Hindlimb; Clonogenic assay

We studied the effect of 30-day hindlimb unloading and subsequent simulated hypergravity on the cellularity and proliferative, clonogenic, and differentiation potential of bone marrow stromal progenitors in mice. Clonogenic and differentiation activity of stromal cells decreased after unloading; proliferative and differentiation activity of bone marrow stromal progenitors increased after hypergravity simulation. Our findings demonstrated negative effect of unloading on functional activity of mouse bone marrow stromal progenitors. Short-term hypergravity after unloading produced a stimulating effect on the bone marrow stromal progenitors.