Combination of Mesenchymal Stromal Cells and Cardiac Stem Cells in a Multilayer Cell Construct Promotes Activation of Notch Signaling and Initiation of Endothelial Differentiation

• DOI: 10.1007/s10517-019-04390-7
• Publication Date: 2019-02-19T13:10:46Z
• Authors: E. K. Shevchenko; K. V. Dergilev; Z. I. Tsokolaeva; I. B. Beloglazova; Yu. D. Molokotina; E. V. Parfenova; M. Yu. Men’shikov
• Keywords: 0301 basic medicine; Tissue Engineering; Myocardial Infarction; Cell Differentiation; Mesenchymal Stem Cells; Rats; Repressor Proteins; 03 medical and health sciences; Basic Helix-Loop-Helix Transcription Factors; Animals; Transcription Factor HES-1; Myocytes, Cardiac; Cells, Cultured; Cell Proliferation; Signal Transduction

We showed the possibility of generating combined tissue-engineered cell consisting of layers of rat cardiac stem cells and mesenchymal stromal cells from the adipose tissue. Cell-cell interaction within the cell sheet promoted proliferation of cardiac stem cells, expression of endothelial differentiation marker ETS1, and Notch signaling activation. The obtained results provide new insights into possible mechanisms of stimulation of endogenous regeneration processes after myocardial damage and demonstrate potential of cell-based cardiomyoplasty with the use of these combined cell sheets.