Secretome of Cultured Human Endothelial Cells in Simulated Microgravity

• DOI: 10.1007/s10517-019-04454-8
• Publication Date: 2019-06-08T07:02:35Z
• Authors: D. N. Kashirina; A. S. Kononikhin; I. M. Larina; L. B. Buravkova
• Keywords: rho GTP-Binding Proteins; Actin Cytoskeleton; 0303 health sciences; 03 medical and health sciences; Human Umbilical Vein Endothelial Cells; Humans; Microtubules; Mass Spectrometry; Weightlessness Simulation; Signal Transduction

The secretome of human umbilical vein endothelial cells (HUVEC) cultured under static conditions and in modeled microgravity for 24 h was studied by chromatography-mass spectrometry. In the secretome of cells exposed to microgravity, we identified a group of microtubule proteins including many structural elements of microtubules and regulatory proteins interacting with Rho-GTPases. Hence, reorganization of actin cytoskeleton and microtubules induced by microgravity is under complex regulation mediated by Rho proteins.