Positive Neuroprotective Effect of Argon Inhalation after Photochemically Induced Ischemic Stroke Model in Rats

• DOI: 10.1007/s10517-024-05984-6
• Publication Date: 2024-01-08T13:02:21Z
• Authors: D. N. Silachev; E. A. Boeva; E. I. Yakupova; M. A. Milovanova; L. A. Varnakova; S. N. Kalabushev; V. V. Antonova; R. A. Cherpakov; I. A. Ryzhkov; K. N. Lapin; M. A. Lyubomudrov; O. A. Grebenchikov
• Keywords: Rats, Sprague-Dawley; Stroke; Oxygen; Neuroprotective Agents; Ischemia; Brain Injuries; Animals; Rats, Wistar; Argon; Rats; Ischemic Stroke; Brain Ischemia

We studied the effect of 2-h inhalation of argon-oxygen mixture (Ar 70%/O2 30%) after photochemically induced stroke and on days 2 and 3 after stroke modeling on the severity of neurological deficit and brain damage (by MRI data) in Wistar rats. Neurological deficit was assessed within 14 days using the limb placement test. MRI and histological study of the brain with an assessment of the size of damage were performed on day 14 after ischemia. Significant differences were obtained in limb placement scores on days 3, 7, and 14, as well as in the volume of ischemic focus by MRI in comparison with the control (ischemia+N2 70%/O2 30%). Inhalation of argon-oxygen mixture for 2 h a day over 3 days after photoinduced stroke decreased the volume of brain damage by 2 times and reduced the severity of neurological deficit.