The protein cargo of extracellular vesicles correlates with the epigenetic aging clock of exercise sensitive DNAmFitAge

• DOI: 10.1007/s10522-024-10177-9
• Publication Date: 2025-01-08T12:28:44Z
• Authors: Bernadett György; Réka Szatmári; Tamás Ditrói; Ferenc Torma; Krisztina Pálóczi; Mirjam Balbisi; Tamás Visnovitz; Erika Koltai; Péter Nagy; Edit I. Buzás; Steve Horvath; Zsolt Radák
• Keywords: Extracellular Vesicles; Aerobic Exercise

Abstract Extracellular vesicles (EVs) are implicated in inter-organ communication, which becomes particularly relevant during aging and exercise. DNA methylation-based clocks reflect lifestyle environmental factors, while regular exercise is known to induce adaptive responses, including epigenetic adaptations. Twenty individuals with High-fitness (aged 57.7 ± 9.8 years) twenty Medium–Low-fitness 57.5 9.7 subjects provided blood samples. EVs were isolated from the samples using a size exclusion chromatography (SEC)-based method, their protein content was analyzed by mass spectrometry (MS). Acceleration of biological age estimator DNAmFitAge (AgeAccelFit) associated cargo EVs, whereas PhenoAge GrimAge acceleration did not show significant relationship. This finding suggests that aging-modulating role may involve communication via EVs. Set Enrichment Analysis performed identify enriched Gene Ontology (GO) terms for sets proteins either correlated AgeAccelFit or detected exclusively high levels aerobic fitness. The further influences inflammation, immune system, cellular repair, adhesion, metabolism coagulation. Our findings help understand preventive exercise, could be mediated part