Polymorphism of the Porcine Leptin Gene Promoter and Analysis of Its Association with Gene Expression and Fatness Traits

• DOI: 10.1007/s10528-006-9070-x
• Publication Date: 2007-02-20T17:22:23Z
• Authors: M. Stachowiak; M. Mackowski; Z. Madeja; M. Szydlowski; A. Buszka; P. Kaczmarek; B. Rubis; P. Mackowiak; K. W. Nowak; M. Switonski
• Keywords: Leptin; 2. Zero hunger; 0303 health sciences; Polymorphism, Genetic; Base Sequence; Swine; Body Weight; Molecular Sequence Data; 03 medical and health sciences; Phenotype; Quantitative Trait, Heritable; Adipose Tissue; Gene Expression Regulation; Gene Frequency; Haplotypes; Animals; Promoter Regions, Genetic

We describe for the first time a 245 bp fragment of the porcine leptin gene promoter in the proximity of the transcription start site. Altogether, 720 pigs were screened with the PCR-SSCP technique for polymorphism in this region. Four SNPs, segregating as two haplotypes, have been identified, one of them (C113G) in the putative consensus site for the AP-2 transcription factor. This polymorphism was evenly distributed in the Duroc breed (n=21) and was absent in the Polish Landrace (n=248) and Pietrain breed (n=12). In the Polish Large White (n=191) and synthetic line 990 (n=243), allele G occurred with a very low frequency. The investigation was performed to test if the C113G SNP affects leptin mRNA level in subcutaneous fat and leptin protein concentration in serum. Additionally, the effect of this polymorphism on fatness traits was statistically analyzed. Although there was a trend toward decreased expression in GG animals, the differences were not significant between genotypes. We also found no evidence for an association of the LEP promoter genotype with the analyzed fatness traits.