Genetic Polymorphisms Along with Dietary and Environmental Factors Enhance the Susceptibility to Nasopharyngeal Carcinoma in Nagaland of Northeast India

• DOI: 10.1007/s10528-020-09954-1
• Publication Date: 2020-06-18T19:02:41Z
• Authors: Chenole Keppen; Prajjalendra Barooah; Parikhit Borthakur; Snigdha Saikia; Manab Deka; Sahana Bhattacharjee; Joshua Keppen; Vinotsole Khamo; Subhash Medhi
• Keywords: Adult; Aged, 80 and over; Male; Inhalation Exposure; Nasopharyngeal Carcinoma; DNA Repair; India; Nasopharyngeal Neoplasms; Middle Aged; Polymorphism, Single Nucleotide; Diet; 3. Good health; 03 medical and health sciences; 0302 clinical medicine; Haplotypes; Risk Factors; Case-Control Studies; Humans; Female; Genetic Predisposition to Disease; Cooking; Genetic Association Studies; Aged

This study investigated the association of seven widely known DNA repair gene polymorphisms (hOGG1 Ser326Cys, XRCC1 Arg194Trp, XRCC1 Arg280His, XRCC1 Arg399Gln, XPC Val499Ala, XPD Lys751Gln and ERCC1 Cys8092Ala) with dietary and environmental factors for Nasopharyngeal Carcinoma (NPC) susceptibility in Nagaland of Northeast India. The genotypes were determined in 128 NPC patients and 180 healthy controls by PCR-RFLP. XRCC1 Arg280His, XPC Val499Ala and ERCC1 Cys8092Ala were found to be associated with NPC risk. Tobacco smoking and burning of firewood for cooking were also found to be a risk factor for NPC. The haplotype analysis of five single-nucleotide polymorphisms (SNPs) XRCC1 Arg194Trp, XRCC1 Arg280His, XRCC1 Arg399Gln, XPD Lys751Gln and ERCC1 Cys8092Ala identified haplotype TGAAC to be significantly associated with NPC. Multifactor dimensionality reduction (MDR) analysis suggested ERCC1 Cys8092Ala to be the best one-factor model that could predict NPC risk. From this study, we conclude that examining the synergistic interactions of various gene-environmental factors together is a better approach to understand NPC susceptibility, instead of their individual effects.