Low salt medium for lactate and ethanol production by recombinant Escherichia coli B

• DOI: 10.1007/s10529-006-9252-y
• Publication Date: 2006-12-11T09:43:19Z
• Authors: Alfredo Martinez; T. B. Grabar; K. T. Shanmugam; L. P. Yomano; S. W. York; L. O. Ingram
• Keywords: Recombination, Genetic; 0106 biological sciences; Bioreactors; Dose-Response Relationship, Drug; Ethanol; Cell Culture Techniques; Escherichia coli; Salts; Lactic Acid; 01 natural sciences; Culture Media; 3. Good health

Individual nutrient salts were experimentally varied to determine the minimum requirements for efficient L (+)-lactate production by recombinant strains of Escherichia coli B. Based on these results, AM1 medium was formulated with low levels of alkali metals (4.5 mM and total salts (4.2 g l(-1)). This medium was equally effective for ethanol production from xylose and lactate production from glucose with average productivities of 18-19 mmol l(-1) h(-1) for both (initial 48 h of fermentation).