Expression of human apolipoprotein A-I in Nicotiana tabacum

• DOI: 10.1007/s10529-010-0388-4
• Publication Date: 2010-09-04T10:36:24Z
• Authors: Pasquale Chiaiese; Maria Minutolo; Angela Arciello; Fulvio Guglielmini; Renata Piccoli; Edgardo Filippone
• Keywords: Nicotiana; 0301 basic medicine; Apolipoprotein A-I; Blotting, Western; Cell Culture Techniques; Gene Expression; Plants, Genetically Modified; Chromatography, Affinity; Recombinant Proteins; 3. Good health; Plant Leaves; 03 medical and health sciences; Humans

Several transgenic tobacco lines expressing human apolipoprotein A-I (ApoA-I) were obtained. Western blot analyses indicated the expression of the recombinant protein in plant organs at various stages of development, including senescent leaves. A cell line expressing human ApoA-I was established from a T(1) transgenic plant. Recombinant ApoA-I was isolated either from extracts of transgenic leaves and from the culture medium of transgenic cells using an antibody-based one-step procedure.