Determination of ionophore antibiotics nactins produced by fecal Streptomyces from sheep

• DOI: 10.1007/s10534-014-9709-8
• Publication Date: 2014-02-04T09:00:38Z
• Authors: Jun Wang; Hongming Tan; Yu Lu; Lixiang Cao
• Keywords: Feces; 0303 health sciences; 03 medical and health sciences; Sheep; Molecular Structure; Animals; Secondary Metabolism; Streptomyces; Anti-Bacterial Agents

To investigate the correlation between fecal actinobacteria and host animals, Streptomyces was isolated from fresh faeces of healthy sheep and secondary metabolites were analyzed. The most frequently isolated strain S161 with antibiotic activity against bacteria and fungi were analyzed. The S161 showed the highest 99 % similarity to Streptomyces canus DSB17 based on the 16S rRNA gene sequence analysis. Metabolite analysis based on MS and NMR spectra showed that S161 produces nactins, cyclotetralactones derived from nonactic acid and homononactic acid as building units of ionophoretic character. Due to ionophores are antimicrobial compounds that are commonly fed to ruminant animals to improve feed efficiency, stable beneficial interactions between Streptomyces bacteria and vertebrates have been demonstrated.