Characterization of zinc amino acid complexes for zinc delivery in vitro using Caco-2 cells and enterocytes from hiPSC

• DOI: 10.1007/s10534-017-0033-y
• Publication Date: 2017-07-17T13:49:17Z
• Authors: Ann Katrin Sauer; Stefanie Pfaender; Simone Hagmeyer; Laura Tarana; Ann-Kathrin Mattes; Franziska Briel; Sébastien Küry; Tobias M. Boeckers; Andreas M. Grabru...
• Keywords: Caco-2; Acrodermatitis enteropathica; Enterocyte

Zn is essential for growth and development. The bioavailability of affected by several factors such as other food components. It therefore interest, to understand uptake mechanisms delivering compounds identify ways bypass the inhibitory effects these factors. Here, we studied effect amino acid conjugates (ZnAAs) on bioavailabilty Zn. We used Caco-2 cells enterocytes differentiated from human induced pluripotent stem a control Acrodermatitis enteropathica (AE) patient, performed fluorescence based assays, protein biochemistry atomic absorption spectrometry characterize cellular ZnAAs. results show that ZnAAs are taken up AA transporters, leading an intracellular enrichment mostly uninhibited antagonists. Enterocytes AE patients were unable gain significant through exposure ZnCl2 but did not differences with respect conclude may possess advantage over classical supplements salts, they be able increase Zn, more efficient in AE.