Indexing and production of virus-free chrysanthemums

• DOI: 10.1007/s10535-005-0152-0
• Publication Date: 2005-03-15T20:40:15Z
• Authors: R. Ram; N. Verma; A. K. Singh; L. Singh; V. Hallan; A. A. Zaidi
• Keywords: 0106 biological sciences; 01 natural sciences; 3. Good health

Chrysanthemum morifolium cv. Regol Time plants were found to be infected with B carlavirus (CVB). They made CVB-free by using meristem tip culture, chemotherapy and thermotherapy. The indexed biological assay, double antibody sandwitch enzyme linked immunosorbent assay (DAS-ELISA) reverse transcriptase polymerase chain reaction (RT-PCR). maximum number of virus-free (26.7 %, as RT-PCR) was obtained 2-thiouracil at 0.04 g dm-3 concentration. Only 12 % virus-free, after being kept 38 °C for 30 d. For indexing CVB in chrysanthemums, RT-PCR the most reliable method.