Induction of tetraploidy in Juncus effusus by colchicine

• DOI: 10.1007/s10535-010-0117-9
• Publication Date: 2010-09-22T09:52:47Z
• Authors: L. Xu; U. Najeeb; M. S. Naeem; M. K. Daud; J. S. Cao; H. J. Gong; W. Q. Shen; W. J. Zhou
• Keywords: Juncus; Colchicine; Epidermis (zoology)

Tetraploidy was induced in vitro mat rush (Juncus effusus L.) cultivar Nonglin-4 by exposure to colchicine (0, 50, 100 and 500 mg dm-3) for 6, 12 24 h. Flow cytometric analysis used confirm the ploidy level. Anatomical ultrastructural analyses at cellular subcellular levels tetraploid diploid control plants revealed differences between plants. The leaf epidermis had larger stomata but lower stomatal density In addition, mesophyll cells appeared more compact showed less intercellular spaces along with increased size of vascular bundles. However, a significant reduction chlorophyll content observed that might be result structural modification lamellar membranes chloroplasts.