The influence of the N- and C- terminal modifications of Potato virus X coat protein on virus properties

• DOI: 10.1007/s10535-012-0130-2
• Publication Date: 2012-03-26T11:30:19Z
• Authors: H. Hoffmeisterova; T. Moravec; H. Plchova; J. Folwarczna; N. Cerovska
• Keywords: Potato virus X; Movement protein; Chimera (genetics); Viral life cycle; C-terminus

The Potato virus X (PVX)-based vector was used for the construction of N- and C-terminally modified PVX coat protein (XCP) chimeras. N-terminal XCP modifications do not influence viral life cycle, whereas simple C-terminal fusion impedes replication. We designed several chimeras tested their viabilities in various Nicotiana benthamiana genotypes. Our results showed negative impact 3'-terminal modification on chimera's cycle. To ensure chimeric constructs stability, second copy last 60 nucleotides followed by 3'-untranslated region (UTR) added downstream recombinant sequence. Simultaneously, first mutated order to prevent recombination between two identical sequences. movement Tobacco mosaic expressed transgenic N. plants positively affected cell-to-cell spread