Identification of phospholipase D genes in Brassica napus and their transcription after phytohormone treatment and pathogen infection

• DOI: 10.1007/s10535-015-0513-2
• Publication Date: 2015-04-28T11:15:25Z
• Authors: M. Janda; L. Ježková; M. Nováková; O. Valentová; L. Burketová; V. Šašek
• Keywords: Brassica rapa; Jasmonate; Methyl jasmonate; Sclerotinia Sclerotiorum; Jasmonic acid; Phospholipase D; Brassicaceae

Phospholipase D (PLD) and its product phosphatidic acid are now considered to be one of the key elements numerous physiological processes in plants including salicylic signalling pathway. The presented study investigates transcriptional regulation Brassica napus PLDs following treatments with defense-related stimuli. We cloned eight B. genes encoding members PLDβ, γ, δ isoforms performed phylogenetic analysis ancestor species rapa oleracea, model plant Arabidopsis thaliana. Transcription identified was monitored after treatment benzothiadiazole (BTH), methyl jasmonate (MeJA), bacterial elicitor flg22, wounding, infection fungal pathogens Sclerotinia sclerotiorum Leptosphaeria maculans. Most responded specifically a particular treatment. Remarkably PLDγ PLDβ were up-regulated by stimuli associated generality this finding confirmed public data from