CsWRKY2, a novel WRKY gene from Camellia sinensis, is involved in cold and drought stress responses

• DOI: 10.1007/s10535-016-0618-2
• Publication Date: 2016-04-14T07:59:28Z
• Authors: Y. Wang; Z. Shu; W. Wang; X. Jiang; D. Li; J. Pan; X. Li
• Keywords: WRKY protein domain; Camellia oleifera

One of the largest families transcriptional regulators contains WRKY proteins. They play important roles in plant defense responses. In this study, a novel gene, CsWRKY2, was isolated from tea [Camellia sinensis (L.) O. Kuntze] plant. The full-length cDNA CsWRKY2 2 050 bp length and encoded 522-amino acid peptide chain containing two typical domains zinc finger motifs, suggesting that member group I family. A subcellular localization assay shows localized to nucleus. Real time qPCR analysis expression higher leaves than other organs induced by cold (4 °C), drought stress, exogenous abscisic (ABA). Additionally, ABA content enhanced after or stress effects were relieved an biosynthesis inhibitor. Furthermore, up-regulated under stresses down-regulated Our findings indicate played role responses participating signaling pathway, downstream ABA.