OsNOX3, encoding a NADPH oxidase, regulates root hair initiation and elongation in rice

• DOI: 10.1007/s10535-018-0814-3
• Publication Date: 2018-06-09T06:47:34Z
• Authors: S. S. Wang; X. N. Zhu; J. X. Lin; W. J. Zheng; B. T. Zhang; J. Q. Zhou; J. Ni; Z. C. Pan; S. H. Zhu; W. N. Ding
• Keywords: Root hair; Elongation

Root hairs play important roles in plant nutrient and water acquisition. To better understand the genetic mechanism controlling root hair development rice (Oryza sativa L.), a mutant with defects was isolated characterized. Cryo-scanning electron microscope (SEM) showed that density length of were significantly reduced compared to wild type (WT). Map-based cloning complementation test revealed mutation occurred NADPH oxidase gene OsNOX3 (LOC_Os01g61880). The displays high sequence similarity previously characterized NOX genes RTH5 maize RHD2 Arabidopsis, which critical development. Expression pattern analysis indicated is expressed various tissues throughout expression roots hairs. Subcellular localization confirmed located on plasma membrane. Staining assays content superoxide hydrogen peroxide are tips Osnox3 when WT. Our results regulating both initiation elongation rice, similar but different from RHD2, confirming difference mechanisms morphogenesis monocot dicot plants.