Glycerol treatment as recovery procedure for cryopreserved human skin allografts positive for bacteria and fungi

• DOI: 10.1007/s10561-011-9244-6
• Publication Date: 2011-03-01T19:36:14Z
• Authors: Gilbert Verbeken; Gunther Verween; Daniel De Vos; Bruno Pascual; Peter De Corte; Cornelia Richters; Arlette De Coninck; Diane Roseeuw; Nadine Ectors; Thomas Rose; Serge Jennes; Jean-Paul Pirnay
• Keywords: Granulation tissue; Human skin; Tissue bank

Human donor skin allografts are suitable and much used temporary biological (burn) wound dressings. They prepare the excised bed for final autografting form an excellent substrate revascularisation formation of granulation tissue. Two preservation methods, glycerol cryopreservation, commonly by tissue banks long-term storage grafts. The burn surgeons Queen Astrid Military Hospital preferentially use partly viable cryopreserved allografts. After mandatory 14-day bacterial mycological culture, however, approximately 15% cannot be released from quarantine because positive culture. To maximize our scarce precious skin, we developed a glycerolisation-based recovery method these culture inactivation method, described in this paper, allowed efficient colonising bacteria fungi, with exception spore-formers, did not influence structural functional aspects