Silencing GTSE-1 expression inhibits proliferation and invasion of hepatocellular carcinoma cells

• DOI: 10.1007/s10565-016-9327-z
• Publication Date: 2016-05-29T23:14:56Z
• Authors: Lei Guo; Shumin Zhang; Bo Zhang; Wanyong Chen; Xiaoqiang Li; Wentao Zhang; Chenhao Zhou; Jubo Zhang; Ning Ren; Qinghai Ye
• Keywords: Male; 0301 basic medicine; Mice, Inbred BALB C; Carcinoma, Hepatocellular; Health, Toxicology and Mutagenesis; Liver Neoplasms; Cell Biology; Toxicology; 3. Good health; Gene Expression Regulation, Neoplastic; Mice; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Animals; Heterografts; Humans; Original Article; RNA, Messenger; Phosphorylation; Microtubule-Associated Proteins; Proto-Oncogene Proteins c-akt; Cell Proliferation; Signal Transduction

G2 and S phase-expressed-1 (GTSE1) was recently reported to upregulate in several types of human cancer, based on negatively regulate p53 expression. However, its expression functional roles hepatocellular carcinoma (HCC) remain unknown. In this study, GTSE1 observed be highly expressed HCC specimens cell lines both at messenger RNA (mRNA) protein levels. Furthermore, high positively associated with tumor size, venous invasion, advanced stage, short overall survival. Moreover, we generated stable knockdown explore the effects silencing growth invasion vitro. determining pathway through which regulated proliferation found inhibit AKT phosphorylation downregulated cycle-related protein. addition, downregulation decreased xenografts. conclusion, these results indicated for first time that overexpression involved progress HCC, enhancing promoting cells.