Fbxo22 inhibits metastasis in triple-negative breast cancer through ubiquitin modification of KDM5A and regulation of H3K4me3 demethylation

• DOI: 10.1007/s10565-022-09754-w
• Publication Date: 2022-09-16T06:03:09Z
• Authors: Siqiaozhi Li; Jinsong He; Xin Liao; Yixuan He; Rui Chen; Junhui Chen; Sean Hu; Jia Sun
• Keywords: H3K4me3; Triple-negative breast cancer; DNA demethylation

Abstract The importance of Fbxo22 in carcinogenesis has been highly documented. Here, we discussed downstream regulatory factors TNBC. RNA-sequencing was conducted for identifying differentially expressed genes, followed by construction a network. Expression patterns Fbxo22/KDM5A TNBC were determined their correlation with the prognosis analyzed. Then, regulation mechanisms between and KDM5A as well H3K4me3 assayed. After silencing overexpression experiments, significance repressing tumorigenesis vitro vivo explored. poorly expressed, while Patients elevated Fbxo22, decreased KDM5A, or higher p16 had long overall survival. reduced levels ubiquitination. promoted histone demethylation to downregulate expression. expression enhance p16, thus inducing DNA damage reducing metastasis Our study validated FBXO22 tumor suppressor through ubiquitination p16.