RNF112, whose transcription is regulated by KLF4, inhibits colorectal cancer growth via promoting ubiquitin-dependent degradation of NAA40

• DOI: 10.1007/s10565-024-09977-z
• Publication Date: 2025-01-06T04:47:00Z
• Authors: Chunfei Li; Wenzheng Guan; Donghua Geng; Yong Feng
• Keywords: KLF4; Deubiquitinating enzyme; Ring finger

RING finger protein 112 (RNF112) exerts a key role in human tumors. However, its biological function colorectal cancer (CRC) has not been discussed. We aimed to explore the and molecular mechanism of RNF112 CRC. In this study, expression was notably decreased CRC tissues cells. Clinical analysis revealed significant association between low tumor size, N classification TNM stage. vitro experiments demonstrated that overexpression repressed cell viability, promoted cycle arrest apoptosis, while knocking down had opposite function. The formation results nude mice supported exerted anti-tumor effects by inhibiting growth promoting apoptosis. Mechanistically, Krüppel-like factor 4 (KLF4) acted as an upstream regulator mediating transcription. Furthermore, we explored downstream discovered it ubiquitination degradation oncoprotein N-alpha-acetyltransferase 40 (NAA40) through ubiquitin ligase activity. addition, NAA40 eliminated effect on tumorigenesis. summary, our findings confirm RNF112, whose transcription is regulated KLF4, inhibits ubiquitin-dependent NAA40. have unraveled KLF4-RNF112-NAA40 axis CRC, which shed light therapeutic strategies for disease.