Paper sludge saccharification for batch and fed-batch production of bacterial cellulose decorated with magnetite for dye decolorization by experimental design

• DOI: 10.1007/s10570-023-05545-6
• Publication Date: 2023-10-31T16:02:11Z
• Authors: Ahmed K. Saleh; Ahmed Salama; Ahmed S. Badawy; Mohamed A. Diab; Hamada El-Gendi
• Keywords: Cellulosic ethanol; Bacterial Cellulose

Abstract Cellulosic wastes represent a great environmental challenge, with potential conversion to product-added value through microbial fermentation. Currently, bacterial cellulose (BC) is considered promising natural polymer for multiple applications. However, the high production cost challenges its wide application. Hence, current study evaluated applicability of paper sludge as cost-effective medium both cellulases and BC production. The local isolate Streptomyces rochei revealed highest cellulase titer (about 3 U/mL) at optimized conditions. For production, batch fed-batch fermentation strategies were using enzymatically hydrolyzed sludge. results asserted advantage advanced (3.10 g/L) over (1.06 under same cultivation developed membranes characterized different instrumental analyses, which an increase in fiber diameters crystallinity Furthermore, BC/magnetite (BC/Fe O 4 ) nanocomposite was by in-situ approach. newly composite dye removal applications, methyl orange (MO) model. conditions Box Behnken design (BBD), indicated maximal MO (83.5%) pH 3.0 BC/Fe concentration 0.1 mg/dL after 60 min. Therefore, asserts good capacity decolorization. paves way implementation removal. Graphical