The Protective Effect of Myo-inositol on Hippocamal Cell Loss and Structural Alterations in Neurons and Synapses Triggered by Kainic Acid-Induced Status Epilepticus

• DOI: 10.1007/s10571-013-9930-y
• Publication Date: 2013-04-08T06:14:11Z
• Authors: Nato Kotaria; Maia Kiladze; Mzia G. Zhvania; Nadezhda J. Japar...; Tamar Bikashvili; Revaz O. Solomonia; Tamar Bolkvadze
• Keywords: Male; Neurons; Analysis of Variance; Kainic Acid; Cell Count; Dendrites; Hippocampus; Rats; 03 medical and health sciences; Neuroprotective Agents; Status Epilepticus; 0302 clinical medicine; Synapses; Animals; Rats, Wistar; Cerebrum; Inositol

It is known that myo-inositol pretreatment attenuates the seizure severity and several biochemical changes provoked by experimentally induced status epilepticus. However, it remains unidentified whether such properties of myo-inositol influence the structure of epileptic brain. In the present light and electron microscopic research we elucidate if pretreatment with myo-inositol has positive effect on hippocampal cell loss, and cell and synapses damage provoked by kainic acid-induced status epilepticus. Adult male Wistar rats were treated with (i) saline, (ii) saline + kainic acid, (iii) myo-inositol + kainic acid. Assessment of cell loss at 2, 14, and 30 days after treatment demonstrate cytoprotective effect of myo-inositol in CA1 and CA3 areas. It was strongly expressed in pyramidal layer of CA1, radial and oriental layers of CA3 and in less degree-in other layers of both fields. Ultrastructural alterations were described in CA1, 14 days after treatment. The structure of neurons, synapses, and porosomes are well preserved in the rats pretreated with myo-inositol in comparing with rats treated with only kainic acid.