Hck Promotes Neuronal Apoptosis Following Intracerebral Hemorrhage

• DOI: 10.1007/s10571-016-0365-0
• Publication Date: 2016-04-06T07:03:41Z
• Authors: Jun Wang; Rongrong Chen; Xiaojuan Liu; Jianhong Shen; Yaohua Yan; Yilu Gao; Tao Tao; Jiansheng Shi
• Keywords: Male; Neurons; Rats, Sprague-Dawley; 0301 basic medicine; 03 medical and health sciences; Cell Line, Tumor; Proto-Oncogene Proteins c-hck; Animals; Humans; Apoptosis; Cerebral Hemorrhage; Rats

The hematopoietic cell kinase (Hck) is a member of the Src family protein kinases which regulates many signal transduction pathways including cell growth, proliferation, differentiation, migration, and apoptosis. However, the expression and function of Hck after intracerebral hemorrhage (ICH) are unknown. Western blot, immunohistochemistry, and immunofluorescence showed that Hck was obviously up-regulation in neurons adjacent to the hematoma after ICH. In addition, the temporary raise of Hck expression was paralleled with the expression of p53, Bax, and active caspase-3, suggesting that Hck was involved in neuronal apoptosis. Hck siRNA dramatically decrease hemin-induced expression of p53, Bax, and active caspase-3 as well as the amount of apoptotic SH-SY5Y cells in vitro. Furthermore, Hck interacted with p53. Hence, Hck might promote neuronal apoptosis via p53 signaling pathway after ICH.