TMEM16A Activation Inhibits Autophagy in Dorsal Root Ganglion Cells, Which is Associated with the p38 MAPK/mTOR Pathway

• DOI: 10.1007/s10571-024-01507-z
• Publication Date: 2024-12-04T07:44:28Z
• Authors: Shuyun Yang; Hui Shang; Yuruo Zhang; Jingsong Qiu; Zheyi Guo; Yong Ma; Yuhang Lan; Shaoyang Cui; Hongshuang Tong; Guocai Li
• Keywords: Dorsal root ganglion

Transmembrane member 16A (TMEM16A) exhibits a negative correlation with autophagy, though the underlying mechanism remains elusive. This study investigates between TMEM16A and autophagy by inducing in DRG neuronal cells using Rapamycin. Results indicated that interference augmented cell viability reduced Rapamycin-induced apoptosis. Autophagosome formation increased but decreased upon overexpression. A similar increase autophagosomes was observed SB203580 treatment. Furthermore, suppressed gene protein expression of p38 MAPK mTOR, whereas overexpression had opposite effect. These findings suggest activation inhibits cells, which is associated MAPK/mTOR pathway, offering potential target for mitigating neuropathic pain (NP).